產(chǎn)品編號(hào) | bsm-33012M-AP |
英文名稱 | Mouse Anti-T7-tag/AP Conjugated antibody |
中文名稱 | 堿性磷酸酶(AP)標(biāo)記的T7-tag(標(biāo)簽)單克隆抗體 |
別 名 | T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag. |
規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
說 明 書 | 100ul |
產(chǎn)品類型 | 標(biāo)簽抗體 |
研究領(lǐng)域 | |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號(hào) | 3G7 |
交叉反應(yīng) | Recombinant protein |
產(chǎn)品應(yīng)用 | WB=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide of T7-tag |
亞 型 | IgG |
純化方法 | affinity purified by Protein G |
儲(chǔ) 存 液 | Constituents: 0.05M TBS, pH 8.0 with 10mg/ml BSA and 0.05% NaN3, 50% glycerol. Or Lyophilized. Buffer = 0.05M TBS, pH 8.0 with 10mg/ml BSA and 0.05% NaN3. Reconstitute with sterile distilled water. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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