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Rabbit Anti-Mus81/BF555 Conjugated antibody (bs-17869R-BF555)
訂購熱線:400-901-9800
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訂購QQ:  400-901-9800
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-17869R-BF555
英文名稱 Rabbit Anti-Mus81/BF555 Conjugated antibody
中文名稱 BF555標(biāo)記的核酸內(nèi)切酶同源蛋白MUS81抗體
別    名 Crossover junction endonuclease MUS81; FLJ21012; FLJ44872; Mus 81; MUS81; MUS81 Endonuclease Homolog; MUS81_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 腫瘤  細(xì)胞生物  細(xì)胞周期蛋白  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) (predicted: Human, Mouse, Rat, Macaque Monkey)
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 61kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Mus81
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Together, DNA repair and checkpoint responses ensure the integrity of the genome. Coordination of cell cycle checkpoints and DNA repair are especially important following genotoxic radiation or chemotherapy, during which unusually high loads of DNA damage are sustained.
MUS81 encodes a helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae (1). Mus81 is important for replicational stress tolerance in both budding and fission yeast.
Specifically, Mus81 associates with Eme1 to form an endonuclease that can process stalled replication forks before they have regressed to form a Holliday junction.
Mus81 associated endonuclease resolves Holliday junctions into linear duplexes by cutting across the junction exclusively on strands of like polarity.
In addition, Mus81 protein abundance increases in cells following exposure to agents that block DNA replication.
Mus81 is involved in the recruitment of Cds1 to aberrant DNA structures where Cds1 modulates the activity of damage tolerance enzymes. The gene encoding human MUS81 maps to chromosome 11q13 and encodes a 551 amino acid protein.

Function:
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Subcellular Location:
Nucleus; nucleolus. Recruited to foci of DNA damage in S-phase cells.

Tissue Specificity:
Widely expressed.

Similarity:
Belongs to the XPF family.
Contains 1 ERCC4 domain.

Database links:

Entrez Gene: 80198 Human

Entrez Gene: 71711 Mouse

Entrez Gene: 293678 Rat

Omim: 606591 Human

SwissProt: Q1JPC1 Cow

SwissProt: Q2KIT9 Cow

SwissProt: Q96NY9 Human

SwissProt: Q91ZJ0 Mouse

SwissProt: Q4KM32 Rat

Unigene: 288798 Human

Unigene: 27697 Mouse

Unigene: 137652 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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