| 產(chǎn)品編號 | bs-13316R-AP |
| 英文名稱 | Rabbit Anti-GCNT2/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的β1-6 N-乙酰氨基葡萄糖轉(zhuǎn)移酶2抗體 |
| 別 名 | bA360O19.2; bA421M1.1; Beta 1 6 N acetylglucosaminyltransferase 2; CCAT; GCNT 2; GCNT2C; GCNT5; Glucosaminyl (N acetyl) transferase 2 I branching enzyme (I blood group); Glucosaminyl (N acetyl) transferase 2 I branching enzyme; I beta 1 6 N acetylglucosaminyltransferase; I branching beta 1 6 acetylglucosaminyltransferase; I branching enzyme; IGNT; II; Ii blood group; MGC163396; N acetylglucosaminyltransferase; N acetyllactosaminide beta 1 6 N acetylglucosaminyltransferase; NACGT1; NAGCT1; ULG3. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 46kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCNT2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Belonging to the glycosyltransferase 14 family, GCNT2 (glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)), also known as II, N-acetylglucosaminyltransferase, IGNT, CCAT, ULG3, GCNT5, GCNT2C or NACGT1, is a 400 amino acid glycosyltransferase that localizes to the Golgi apparatus. Other members of the glycosyltransferase 14 family include GCNT1, GCNT3, GCNT4, GCNT6 and GCNT7. A single-pass type II membrane protein, GCNT2 functions as a branching enzyme known as beta-1,6-N-acetylglucosaminyltransferase, which converts fetal i antigen to adult I antigen in erythrocytes during embryonic development. With expression levels increasing significantly during oncogenesis and development, GCNT2 is found at highest levels in adult prostate and fetal brain, and is found at low levels in heart, small intestine, colon, brain, pancreas and kidney. Function: GCNT2 is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase that converts linear into branched poly-N-acetyllactosaminoglycans and is responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding 3 different isoforms have been described. Subcellular Location: Golgi apparatus membrane. Tissue Specificity: In the adult, highly expressed in prostate and to a lesser extent in small intestine and colon. Barely detected in heart, brain, kidney and pancreas. No expression in placenta, lung, liver, skeletal muscle, spleen, thymus, testis, ovary and peripheral blood leukocytes. In fetus, highly expressed in brain and to a lesser extent in lung and kidney. Barely detected in liver. Similarity: Belongs to the glycosyltransferase 14 family. Database links: Entrez Gene: 2651 Human Entrez Gene: 14538 Mouse Omim: 600429 Human SwissProt: Q8N0V5 Human SwissProt: P97402 Mouse Unigene: 519884 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
