產(chǎn)品編號(hào) | bs-11976R-PE-Cy3 |
英文名稱 | Rabbit Anti-NOTCH1/PE-Cy3 Conjugated antibody |
中文名稱 | PE-Cy3標(biāo)記的Notch1蛋白抗體 |
別 名 | hN1; Neurogenic locus Notch homolog protein 1; activated Notch 1; activated Notch-1;Notch homolog 1 translocation associated (Drosophila); NOTCH1; TAN1; Translocation-associated Notch protein TAN-1; |
規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
說 明 書 | 100ul |
研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 發(fā)育生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | (predicted: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep, ) |
產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 271kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human NOTCH1 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008]. Function: Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. The protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands. Once the Notch extracellular domain interacts with a ligand, a protease called TACE (Tumor Necrosis Factor Alpha Converting Enzyme) cleaves the Notch protein just outside the membrane. This releases the extracellular portion of Notch, which continues to interact with the ligand. The ligand plus the Notch extracellular domain is then endocytosed by the ligand expressing cell. After this first cleavage, an enzyme called gamma-secretase cleaves the remaining part of the Notch protein just inside the inner leaflet of the cell membrane. This releases the intracellular portion of the Notch protein, which then moves to the nucleus and causes various genes to be expressed. There are many other proteins involved in the intracellular portion of the Notch signalling cascade. Subunit: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation Subcellular Location: Cell membrane; Single-pass type I membrane protein. Notch 1 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus Tissue Specificity: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Post-translational modifications: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). Phosphorylated (By similarity). O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose. Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD DISEASE: Defects in NOTCH1 are a cause of aortic valve disease 1 (AOVD1) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome. Similarity: Belongs to the NOTCH family. Contains 5 ANK repeats. Contains 36 EGF-like domains. Contains 3 LNR (Lin/Notch) repeats. Database links: Entrez Gene: 4851 Human Entrez Gene: 18128 Mouse Omim: 190198 Human SwissProt: P46531 Human SwissProt: Q01705 Mouse Unigene: 495473 Human nigene: 290610 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 四川少妇BBw搡BBBB槡BBBB 四川少妇bbw搡bbbb搡bbbb | 色情午夜 码一区二区 | 免费女人高潮又粗肛交毛片 | 日本人做爰毛片免费播 | 欧美精品免费一区欧美久久优播 | 影音先锋av资源网站 | 国产精品久久久久AV | 性一交一乱一色一视频麻豆 | 欧洲无码八A片人妻少妇网站直播 | 午夜理理伦电影A片朋友夫妇 | 羞羞视频最新地址发布页 | 国产精品久久久久久久久久久久无码 | 中文字幕无码人妻av | 亚洲无码高清在线观看 | 97国产精品久久久久久 | 中文字幕一二三区 | 91蜜桃传媒精品久久久一区二区 | 国产无套一区二区三区网站 | 国产成人精品视频A片免费蜜月 | 又粗又大农村妇女A片 | 中出人妻中文字幕一区十八 | 国产精品白丝jk喷白浆软件 | 少妇性BBB搡BBB爽爽爽视頻 | 欧美少妇大奶色交视频 | 日韩中文字幕免费观看一区 | 国产一级a毛一级a看免费视频野外 | 无码毛多爆乳一二三区 | 国产农村乱对白刺激视频 | 五月婷婷一区二区 | 国产亲子伦XXXXX对白 | 青青草91青娱盛宴国产 | 国产成人综合日韩精品无码香 | 少妇精品无码一区二区三区大长颈 | 日本中文字幕在线播放 | 乡下农村妇女一级毛片水多 | 538prom精品视频任你爽 | 国产精品色情免费视频 | 91丨国产丨白浆秘 洗澡动漫 | 成年人免费视频麻豆 | 久久久久成人精品视频 |