產(chǎn)品編號 | bs-5466R-RBITC |
英文名稱 | Rabbit Anti-phospho-JunD (Ser255)/RBITC Conjugated antibody |
中文名稱 | 羅丹明(RBITC)標記的磷酸化活化蛋白激酶D抗體 |
別 名 | JunD (phospho S255); JunD (Phospho Ser255); Transcription factor jun-D; jun D proto-oncogene; JUND1; AP-1; JUND; JUND1; MGC6245; MGC72300; AP 1; AP1; jun D proto oncogene; JunD FL isoform; JUND_HUMAN; Transcription factor jun D; transcription factor jun-D isoform JunD-FL; Jun-like transcription factor; pfam03957. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 磷酸化抗體 |
研究領域 | 腫瘤 免疫學 信號轉導 轉錄調(diào)節(jié)因子 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Chicken, Dog, Cow, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 38kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human JunD around the phosphorylation site of Ser255 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'. Function: Transcription factor binding AP-1 sites. Subunit: Binds DNA as a dimer (By similarity). Interacts with MEN1; this interaction represses transcriptional activation. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain. Database links: Entrez Gene: 3727 Human Entrez Gene: 16478 Mouse Omim: 165162 Human SwissProt: P17535 Human SwissProt: P15066 Mouse Unigene: 2780 Human Unigene: 419851 Mouse Unigene: 46225 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Jun-D屬于轉錄因子AP-1家族(Jun蛋白家族包括c-Jun、JunB和JunD) |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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