產品編號 | bs-1096R-Cy7 |
英文名稱 | Rabbit Anti-SKP2/Cy7 Conjugated antibody |
中文名稱 | Cy7標記的細胞S期激酶相關蛋白2抗體 |
別 名 | p45skp2; skp2p45; p45; skp2 p45;S-phase kinase-associated protein 2; SKP2; CDK2/Cyclin A associated protein p45; Cyclin A/CDK2 associated protein p45; F box protein Skp2; F box/LRR repeat protein 1; FBL 1; FBL1; FBXL 1; FBXL1; FLB 1; FLB1; MGC1366; SKP2_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 信號轉導 激酶和磷酸酶 細胞分化 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, (predicted: Mouse, Rat, Cow, Rabbit, ) |
產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 48kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Skp2 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產品介紹 |
background: Substrate recognition component of a SCF(SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1L, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. [PATHWAY] Protein modification; protein ubiquitination. [SUBUNIT] Part of the SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin A-CDK2 complex. Interacts with ORC1L, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 and MLL. [SIMILARITY] Contains 1 F-box domain. [SIMILARITY] Contains 8 LRR (leucine-rich) repeats. Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration. Subunit: Part of a SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin-A-CDK2 complex. Interacts with ORC1, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1, UBP43, MYC, TOB1, TAL1 and MLL. Interacts with TRIM21. Subcellular Location: Cytoplasm. Nucleus. Post-translational modifications: Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13. Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3. Similarity: Contains 1 F-box domain. Contains 9 LRR (leucine-rich) repeats. Database links: Entrez Gene: 6502 Human Entrez Gene: 27401 Mouse Omim: 601436 Human SwissProt: Q13309 Human SwissProt: Q9Z0Z3 Mouse Unigene: 23348 Human Unigene: 35584 Mouse Unigene: 154278 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Skp2異常表達可加速細胞周期轉化,該蛋白與腫瘤分化程度有關,Skp2參與細胞轉化和腫瘤的形成,目前主要用于消化系統(tǒng)腫瘤方面的研究。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 国产传媒啊啊啊视频 | 911亚洲精品无码成人A片在线 | 337P粉嫩大胆色噜噜嚕动态图 | 国产毛片乡下农村妇女 | 景音先锋日韩欧美制服亚洲 | 午夜影院在线免费观看 | 4444WWW西西大胆高清视频 | 国产成人91一区二区三区APP | 黄色的网站在线免费观看 | 四lll少妇BBBB槡BBBB | 成人在线免费观看视频 | 香蕉大视频一二三区乱码 | 内射少妇39亚洲区 | 午夜福利视频 无码 | 120分钟做爰免费视频 | 中文字幕日产A片在线看 | 毛片免费在线观看视频 | 糟蹋人妻HD中文字幕 | 国产精品乱码一区二区免费视频 | 亚洲欧美动漫偷拍 | 丰满少妇内射完整版 | 黃色视频网站在线观看 | 久久AⅤ乱码一区二区三区 亂倫近親相姦中文字幕网站 | 亚洲精品秘 一区二区三小 91麻豆精品久久久久蜜臀 | 老熟妇一区二区三区啪啪 | 国产成人91亚洲精品无码观看 | 和孩做爰A片免费播放 | 91午夜人妻人人做人爽 | 兴奋视频网站在线观看 | 中文字幕免费观看哔哔在线 | 后入性感美女白浆 | 最近日本中文字幕中文 | 精品秘 无码一区二区久久 无码免费婬AV片在线观看 | 日本青草久久老色鬼 | 在线视频福利你懂的 | 免费一级特黄特色大片 | 久久久久无码精品国产H动漫猫咪 | 午夜福利三级电影 | 成人亚洲A片V一区二区三区蜜月 | av无码在线播放 |