產(chǎn)品編號 | bs-7612R-BF555 |
英文名稱 | Rabbit Anti-HIPK4/BF555 Conjugated antibody |
中文名稱 | BF555標記的同源相互作用蛋白激酶4抗體 |
別 名 | HIPK 4; HIPK4; HIPK4_HUMAN; Homeodomain interacting protein kinase 4; Homeodomain-interacting protein kinase 4. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 細胞凋亡 細胞周期蛋白 激酶和磷酸酶 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 69kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HIPK4 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. The homeodomain-interacting protein kinases (HIPK1, HIPK2, HIPK3 and HIPK4) comprise a subfamily of kinase proteins that have a conserved protein kinase domain, as well as a separate domain that interacts with homeoproteins. HIPK4 (Homeodomain-interacting protein kinase 4) is a 616 amino acid protein that localizes to the cytoplasm and exists as two isoforms that are produced by alternative splicing events. Like other members of the HIPK family, HIPK4 functions as a protein kinase that catalyzes the ATP-dependent phosphorylation of target proteins and is thought to act as a corepressor of target transcription factors. Function: Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors. Subcellular Location: Cytoplasm (By similarity). Post-translational modifications: Autophosphorylated (By similarity). Similarity: Belongs to the protein kinase superfamily. CMGCSer/Thr protein kinase family. HIPK subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 147746 Human Entrez Gene: 233020 Mouse Omim: 611712 Human SwissProt: Q8NE63 Human SwissProt: Q3V016 Mouse Unigene: 79363 Human Unigene: 429716 Mouse Unigene: 41008 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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