| 產(chǎn)品編號 | bs-2350R-BF647 |
| 英文名稱 | Rabbit Anti-PER1 protein/BF647 Conjugated antibody |
| 中文名稱 | BF647標(biāo)記的節(jié)律抑制蛋白PER1抗體 |
| 別 名 | Circadian clock protein PERIOD 1; Circadian clock protein PERIOD1; Circadian pacemaker protein Rigui; hPER 1; hPER; hPER1; KIAA0482; MGC88021; PER 1; PER; PER1; PER1 protein; PER1_HUMAN; Period 1; Period circadian protein homolog 1; Period drosophila homolog of; Period homolog 1; Period1; RIGUI. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 神經(jīng)生物學(xué) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 136kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PER1 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT 3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila PER. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL 1 transactivation complex. Function: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Can bind heme (By similarity). Subunit: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS, PER2, PER3 and, through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts with GPRASP1 (By similarity). Binding to CSNK1G2 triggers proteasomal degradation. Subcellular Location: Nucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2 (By similarity). Tissue Specificity: Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney. Post-translational modifications: Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Ubiquitinated (By similarity). Similarity: Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains. Database links: Entrez Gene: 100731177 Guinea pig Entrez Gene: 100073046 Horse Entrez Gene: 5187 Human Entrez Gene: 18626 Mouse Omim: 602260 Human SwissProt: O15534 Human SwissProt: O35973 Mouse Unigene: 445534 Human Unigene: 7373 Mouse Unigene: 34433 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Per1蛋白的作用是機體生理節(jié)奏調(diào)節(jié)的核心基因，也是影響腫瘤發(fā)生發(fā)展的重要蛋白。調(diào)節(jié)機體生理節(jié)奏、調(diào)控細(xì)胞周期和促進(jìn)DNA損傷修復(fù)等作用。Per1廣泛參與了細(xì)胞的周期調(diào)控、DNA損傷修復(fù)、細(xì)胞凋亡和腫瘤細(xì)胞增殖等過程 |
