| 產(chǎn)品編號(hào) | bs-2495R-FITC |
| 英文名稱 | Rabbit Anti-CD30/FITC Conjugated antibody |
| 中文名稱 | FITC標(biāo)記的CD30抗體 |
| 別 名 | CD 30; CD30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; Tumor Necrosis Factor Receptor Superfamily Member 8. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說(shuō) 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細(xì)胞類型標(biāo)志物 腫瘤細(xì)胞生物標(biāo)志物 細(xì)胞因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Pig, (predicted: Rabbit, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 62kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD30 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. Subunit: Interacts with TRAF1, TRAF2, TRAF3 and TRAF5. Subcellular Location: Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Cytoplasm. Post-translational modifications: Phosphorylated on serine and tyrosine residues. Similarity: Contains 6 TNFR-Cys repeats. Database links: Entrez Gene: 943 Human Omim: 153243 Human SwissProt: P28908 Human Unigene: 1314 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD30是一種單鏈糖蛋白，標(biāo)記活化T和B淋巴細(xì)胞，R-S細(xì)胞和部分淋巴濾泡間區(qū)免疫母細(xì)胞。CD30是霍奇金淋巴瘤的一個(gè)特異性標(biāo)記，也是大細(xì)胞間變型淋巴瘤的診斷型標(biāo)志。 |
| 產(chǎn)品圖片 |
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-TNFRSF8 antibody (bs-2495R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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