| 產品編號 | bs-2471R-BF488 |
| 英文名稱 | Rabbit Anti-ICOS ligand/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的誘導協(xié)同刺激分子配體CD275抗體 |
| 別 名 | B7 H2; B7 homolog 2; B7 homologue 2; B7 like protein Gl50; B7 related protein 1; B7-like protein Gl50; B7-related protein 1; B7-H2; B7H2; B7RP 1; B7RP-1; B7RP1; CD 275; CD275; CD275 antigen; GL 50; GL50; ICOS L; ICOS LG; ICOS ligand; ICOSL; ICOSL_HUMAN; Icoslg; Inducible T cell co stimulator ligand; KIAA0653; LICOS; Transmembrane protein B7 H2 ICOS ligand; ICOSLG; ICOS ligand-like; ICOS ligand. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 免疫學 轉錄調節(jié)因子 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, (predicted: Rat, ) |
| 產品應用 | Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ICOS ligand |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: ICOS ligand is a member of the B7 family and the immunoglobulin superfamily. Human ICOS ligand is expressed by activated monocytes/macrophages and dendritic cells. It binds to a CD28 like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. This interaction plays an important role in the T cell costimulation pathway. Function: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. Subunit: Membrane; Single-pass type I membrane protein. Subcellular Location: Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 23308 Human Entrez Gene: 50723 Mouse Omim: 605717 Human SwissProt: O75144 Human SwissProt: Q9JHJ8 Mouse Unigene: 14155 Human Unigene: 17819 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. B7協(xié)同刺激分子及其相應受體結合所產生的信號在調節(jié)T細胞活化及效應性細胞因子分泌中起重要作用，具有負性調節(jié)作用的協(xié)同刺激分子對免疫應答的適度進行、防止自身免疫疾病的發(fā)生具有重要作用。 B7H2可協(xié)同刺激CD14 + 細胞增殖，并分泌IL-4、IL-5、IFN-β、TNF-α、GM-CSF和IL-10等細胞因子，但不能協(xié)同刺激T細胞上調IL-2的分泌。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
