產(chǎn)品編號(hào) | bs-2339R-PE-Cy7 |
英文名稱 | Rabbit Anti-EV71 polyprotein 3D/PE-Cy7 Conjugated antibody |
中文名稱 | PE-Cy7標(biāo)記的腸道病毒71型/手足口病病毒抗體 |
別 名 | 3D polymerase; Human enterovirus 71 polyprotein 3D; EV71; Enterovirus 71. |
規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
說(shuō) 明 書 | 100ul |
研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)菌及病毒 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | |
產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 351kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from EV71 polyprotein 3D |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Enteroviruses, such as enterovirus 71, are classified to be in the picornavirus family, pico [small] + RNA [ribonucleic acid] + virus. Picornaviruses are among the smallest and simplest ribonucleic acid containing viruses known (1). The RNA for many enteroviruses have now been cloned and complete genomic sequences have been obtained. The RNA from all sequenced enteroviruses are similar in length, about 7400 nucleotides, and have identical organization (1). The human alimentary tract is the predominant site of enterovirus replication and these viruses were first isolated from enteric specimens. These viruses are the cause of paralytic poliomyelitis, aseptic meningitis-encephalitis, myocarditis, pleurodynia, hand-foot-and-mouth disease, conjunctivitis, and numerous other syndromes associated with extra-intestinal target organs. There are 67 numbered types of enteroviruses in the enterovirus family (1): three polioviruses, twenty-three coxsackieviruses A, six coxsackieviruses B, thirty-one echoviruses, and four other enteroviruses. Function: Protein VP1: Forms, together with VP2 and VP3, an icosahedral capsid (pseudo T=3), 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Protein VP1 mainly forms the vertices of the capsid. VP1 interacts with host cell receptor to provide virion attachment to target cell. After binding to its receptor, the capsid undergoes conformational changes. VP1 N-terminus (that contains an amphipathic alpha-helix) is externalized, VP4 is released and together, they shape a virion-cell connecting channel and a pore in the host membrane through which RNase-protected transfer of the viral genome takes place. After genome has been released, the channel shrinks. Protein VP2: Forms, together with VP1 and VP3, an icosahedral capsid (pseudo T=3), 300 Angstroms in diameter, composed of 60 copies of each capsid protein and Protein VP3: Forms, together with VP1 and VP2, an icosahedral capsid (pseudo T=3), 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. VP4 is released, VP1 N-terminus is externalized, and together, they shape a virion-cell connecting channel and a pore in the host membrane through which RNase-protected transfer of the viral genome takes place. After genome has been released, the channel shrinks. Protein VP0: Protein VP0: VP0 precursor is a component of immature procapsids, which gives rise to VP4 and VP2 afer maturation. Allows the capsid to remain inactive before the maturation step. Protease 2A: cysteine protease that is responsible for the cleavage between the P1 and P2 regions. It cleaves the host translation initiation factor EIF4G1, in order to shut off the capped cellular mRNA transcription (By similarity). Protein 2B: Affects membrane integrity and cause an increase in membrane permeability. Protein 2C: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities. Protein 3A, via its hydrophobic domain, serves as membrane anchor. It also inhibits endoplasmic reticulum-to-Golgi transport. Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease. RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals. Subunit: Protein 2C N-terminus interacts with human RTN3. This interaction is important for viral replication. Subcellular Location: Protein VP2: Virion. Host cytoplasm (Potential). Protein VP3: Virion. Host cytoplasm (Potential). Protein VP1: Virion. Host cytoplasm (Potential). Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum. Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum. Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum. Protein 3B: Virion (Potential). Protease 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum. Post-translational modifications: Specific enzymatic cleavages in vivo by the viral proteases yield a variety of precursors and mature proteins. Polyprotein processing intermediates such as VP0 which is a VP4-VP2 precursor are produced. During virion maturation, non-infectious particles are rendered infectious following cleavage of VP0. This maturation cleavage is followed by a conformational change of the particle. VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase. Myristoylation of VP4 is required during RNA encapsidation and formation of the mature virus particle. Similarity: Belongs to the picornaviruses polyprotein family. Contains 2 peptidase C3 domains. Contains 1 RdRp catalytic domain. Contains 1 SF3 helicase domain. Database links: SwissProt: Q66478 HE71B Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 91成人一区二区 | 超碰在线免费成人WWW | 亚洲无码高清视频在线观看 | 高清无码一区二区三区四区 | 成人人人人人欧美片做爰 | 美女扒开尿口让男人桶 | 久久久久久久久久久久久久动漫 | 国产特级婬片免费看 | 国产91 丝袜在线播放导 | 黄色对白视频在线观看 | 免费一级婬片A片AAA毛片肥女 | 风流少妇A片一区二区蜜桃 真实露脸农村妇女23p | 国产日韩免费高清视频 | 欧美一区二区在线观看 | 一级黄片在线免费观看 | 日本熟女性爱视频 | 红杏A片视频网站入口 | 国产乱XXⅩXX国语对白 | 亚洲五码在线观看视频 | 99精品成人无码A片漫画 | 国产凹凸熟女一区二区 | 高清无码免费在线观看 | 一级内射片在线网站观看 | 无码精品人妻一区二区三区蜜桃 | 青青草原视频在线观看永久入口 | 欧美性猛交XXXXX水多 | 啊啊啊射精好爽啊国产视频 | 日本一级特黄大真人片 | 香蕉一级婬片A片久久精 | 成人亚洲A片V一区二区三区蜜月 | 高潮白浆大鸡巴AV | 国产人妻A片三毛片 | 一级毛片久久久久久久女人18 | 一级a免一级a做免费 | 亚洲国产AV诱惑 | 精品99视频免费在线观看 | 成人电影精品国产免费 | 中文区中文字幕免费看 | the Porn日本在线 | 国产无遮挡裸体免费视频 |