產(chǎn)品編號 | bsm-52926R |
英文名稱 | CD171/L1CAM Recombinant Rabbit mAb |
中文名稱 | 神經(jīng)細(xì)胞粘附分子配體1重組兔單抗 |
別 名 | L1CAM_HUMAN; Neural cell adhesion molecule L1; CAML1; MIC5; N-CAM-L1; NCAM-L1; CD171; L1 cell adhesion molecule; S10; HSAS; MASA; SPG1; HSAS1; N-CAM-L1; |
研究領(lǐng)域 | 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 1C3 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 138 kDa |
檢測分子量 | |
細(xì)胞定位 | 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant human L1CAM protein |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
L1cam (L1 cell adhesion molecule isoform 1 precursor) is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq]. SWISS: P32004 Gene ID: 3897 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD171/L1CAM monoclonal antibody, unconjugated (bsm-52926R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD171/L1CAM Monoclonal Antibody, Unconjugated(bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD171/L1CAM Monoclonal Antibody, Unconjugated(bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD171/L1CAM Monoclonal Antibody, Unconjugated(bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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