| 產(chǎn)品編號(hào) | bsm-60428R |
| 英文名稱 | ASGPR Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | H12B5 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:200-1:1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:20-1:100,ICC/IF=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 介導(dǎo)血漿糖蛋白的內(nèi)吞作用，其復(fù)雜碳水化合物部分上的末端唾液酸殘基已被去除。 該受體識(shí)別末端半乳糖和N-乙酰半乳糖胺單元。 配體與受體結(jié)合后，所得復(fù)合物被內(nèi)化并轉(zhuǎn)運(yùn)至分選細(xì)胞器，在該細(xì)胞器中受體與配體解離。 然后受體回到細(xì)胞膜表面。鈣是配體結(jié)合所必需的。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HepG2, 2: Human liver
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 33 kDa
Observed MW: 40-50 kDa
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60428R
Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60428R
Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60428R
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