產(chǎn)品編號(hào) | bsm-60593R |
英文名稱(chēng) | LY75 Recombinant Rabbit mAb |
中文名稱(chēng) | 淋巴細(xì)胞抗原75(CD205)重組兔單抗 |
別 名 | LY75_HUMAN; Lymphocyte antigen 75; CD205; CLEC13B; Ly-75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6; |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Recombinant |
克 隆 號(hào) | D11H12 |
交叉反應(yīng) | Human,Mouse,Rat |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 195 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Predicted to enable signaling receptor activity. Predicted to be involved in immune response and inflammatory response. Located in extracellular exosome. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. Database links: Entrez Gene: 4065 Human SwissProt: O60449 Human LY75/DEC-205是一種介導(dǎo)胞吞作用的多凝集素受體,主要表達(dá)于樹(shù)突狀細(xì)胞和胸腺上皮細(xì)胞表面,屬于巨噬細(xì)胞甘露糖受體家族成員。 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with LY75 monoclonal antibody, unconjugated (bsm-60593R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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