| 產(chǎn)品編號 | bsm-60152R |
| 英文名稱 | Mono-Methyl-Histone H4 (Lys12) Recombinant Rabbit mAb |
| 中文名稱 | |
| 產(chǎn)品類型 | 甲基化抗體 重組兔單抗 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | B11E4 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:50-1:100,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾，也稱作“組蛋白密碼”，通過調(diào)控染色質(zhì)結(jié)構(gòu)發(fā)揮作用，是表觀遺傳學的重要研究分支。 組蛋白上的翻譯后修飾包括乙酰化，甲基化，磷酸化和近年發(fā)現(xiàn)的一些新型?；揎?。這些組蛋白修飾直接影響染色質(zhì)和轉(zhuǎn)錄因子或其他表觀調(diào)控子的結(jié)合，改變基因組的穩(wěn)定性和基因轉(zhuǎn)錄等。組蛋白甲基化通常發(fā)生在核心組蛋白的賴氨酸和精氨酸側(cè)鏈氨基上。組蛋白甲基化既可以促進也可以抑制基因轉(zhuǎn)錄，這取決于組蛋白甲基化是發(fā)生在賴氨酸還是發(fā)生在精氨酸上以及甲基化基團的個數(shù)（賴氨酸可以發(fā)生一、二、三甲基化，精氨酸可發(fā)生單、對稱與非對稱二甲基化）。組蛋白賴氨酸甲基化通常發(fā)生在組蛋白H3第4，9，27，36，79和組蛋白H4的第5, 8, 12, 20位賴氨酸上；精氨酸甲基化通常發(fā)生在組蛋白H3的第2，8，17，26和組蛋白H4第4位精氨酸上。蛋白甲基化酶（HMT）和組蛋白去甲基化酶（HDM）是甲基化修飾主要的調(diào)控因子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1. HeLa, 2. NIH-3T3, 3. BRL, 4. Mouse heart，5. Rat heart, 6. Mouse kidney, 7. Rat kidney, 8. Recombinant Histone H4
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human prostate hyperplasis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60152R
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60152R
Tissue: Rat stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60152R
Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60152R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60152R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
