| 產(chǎn)品編號(hào) | bsm-60276R |
| 英文名稱(chēng) | TNS4 Recombinant Rabbit mAb |
| 中文名稱(chēng) | 張力蛋白4重組兔單抗 |
| 別 名 | C terminal tensin like; C terminal tensin like protein; C-terminal tensin-like protein; CTEN; c-TEN; FLJ14950; PP14434; TENS4_HUMAN; Tensin 4; Tensin-4. |
| 研究領(lǐng)域 | 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | C5F6 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 75 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TNS4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Predicted to enable actin binding activity. Involved in protein localization. Located in focal adhesion. [provided by Alliance of Genome Resources, Nov 2021] SWISS: Q8IZW8 Gene ID: 84951 Database links: Entrez Gene: 84951 Human Entrez Gene: 217169 Mouse SwissProt: Q8IZW8 Human SwissProt: Q8BZ33 Mouse |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at
room temperature
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 76 kDa
Observed MW: 90 kDa
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNS4 Monoclonal Antibody, Unconjugated(bsm-60276R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60276R
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