| 產(chǎn)品編號 | bsm-51741M |
| 英文名稱 | Cyclooxygenase 2 Mouse mAb |
| 中文名稱 | 環(huán)氧合酶2單克隆抗體 |
| 別 名 | PTGS2; COX 2; COX2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 心血管 免疫學 信號轉(zhuǎn)導 合成與降解 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | F4H9 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PTGS2. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] Function: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Subunit: Homodimer. Subcellular Location: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Post-translational modifications: S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. Similarity: Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain. SWISS: P35354 Gene ID: 5743 Database links: Entrez Gene: 5743 Human SwissProt: P35354 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human THP-1(LPS) cell lysates
Lane 2: Human LOVO cell lysates
Primary: Anti-Cyclooxygenase 2 (bsm-51741M) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa
25 ug total protein per lane of various lysates (see on figure) probed with PTGS2/Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-51741M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
