| 產品編號 | bsm-52012R |
| 英文名稱 | Alas1 (1G11) Recombinant Rabbit mAb |
| 中文名稱 | 5-氨基乙酰丙酸合酶1重組兔單抗 |
| 別 名 | mitochondrial; nonspecific; 5 aminolevulinate synthase; 5 aminolevulinate synthase nonspecific mitochondrial; 5 aminolevulinic acid synthase; 5-aminolevulinate synthase; 5-aminolevulinic acid synthase 1; Alas 1; ALAS 3; ALAS; ALAS H; ALAS N; ALAS-H; alaS1; ALAS3; ALASH; Aminolevulinate delta synthase 1; Aminolevulinic acid synthase 1; delta ALA synthetase; delta aminolevulinate synthase; delta-ALA synthase 1; delta-aminolevulinate synthase 1; HEM1_HUMAN; MIG 4; MIG4; Migration inducing protein 4. |
| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1G11 |
| 交叉反應 | Human (predicted: Mouse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | recombinant human Alas1 C-terminal 200aa |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. Subunit: Homodimer. Subcellular Location: Mitochondrion matrix Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. SWISS: P13196 Gene ID: 211 Database links: Entrez Gene: 211 Human Entrez Gene: 11655 Mouse Omim: 125290 Human SwissProt: P13196 Human SwissProt: Q8VC19 Mouse Unigene: 476308 Human Unigene: 290578 Mouse Unigene: 97126 Rat |
| 產品圖片 |
Sample:
Lane 1: JAR (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: MCF-7 (Human) Cell Lysate at 30 ug
Lane 6: K562 (Human) Cell Lysate at 30 ug
Lane 7: Raji (Human) Cell Lysate at 30 ug
Primary: Anti-Alas1 (bsm-52012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71/65 kD
Observed band size: 65 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
