| 產(chǎn)品編號(hào) | bsm-51234M |
| 英文名稱 | MGMT Mouse mAb |
| 中文名稱 | O6甲基鳥(niǎo)嘌呤DNA甲基轉(zhuǎn)移酶單克隆抗體 |
| 別 名 | 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT: O-6 methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6 methylguanine DNA alkyltransferase; O-6-methylguanine-DNA methyltransferase; O6 methylguanine DNA methyltransferase; MGMT_HUMAN. |
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Specific References (2) | bsm-51234M has been referenced in 2 publications.
[IF=6.126] Paukovcekova S et al. Novel Thiosemicarbazones Sensitize Pediatric Solid Tumor Cell-Types to Conventional Chemotherapeutics through Multiple Molecular MechanismsCancers (Basel).2020 Dec 15;12(12):3781. WB ; Human.
[IF=2.348] Ishi Y et al. Prognostic role of H3K27M mutation, histone H3K27 methylation status, and EZH2 expression in diffuse spinal cord gliomas. Brain Tumor Pathol
. 2020 Jul;37(3):81-88. IHC ; Human.
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| 研究領(lǐng)域 | 腫瘤 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 4C12 |
| 交叉反應(yīng) | (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:20-100,IF=1:20-100,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human MGMT |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients. Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Subcellular Location: Nucleus. Similarity: Belongs to the MGMT family. SWISS: P16455 Gene ID: 4255 Database links: Entrez Gene: 4255 Human Omim: 156569 Human SwissProt: P16455 Human Unigene: 501522 Human |
