產(chǎn)品編號 | bsm-33279M |
英文名稱 | Mouse Anti-Bax antibody |
中文名稱 | Bax單克隆抗體 |
別 名 | apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN. |
![]() |
Specific References (8) | bsm-33279M has been referenced in 8 publications.
|
研究領域 | 腫瘤 細胞生物 細胞凋亡 細胞外基質(zhì) |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 8H12 |
交叉反應 | Human,Mouse (predicted: Rat) |
產(chǎn)品應用 | WB=1:1000-1500,IHC-P=1:100-500,IHC-F=1:200-500,IF=1:200-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 21 kDa |
檢測分子量 | |
細胞定位 | 細胞漿 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Bax |
亞 型 | IgG1 |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]. Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Subunit: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Subcellular Location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential). Tissue Specificity: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Similarity: Belongs to the Bcl-2 family. SWISS: Q07812 Gene ID: 581 Database links: Entrez Gene: 581 Human Entrez Gene: 12028 Mouse Omim: 600040 Human SwissProt: Q07812 Human SwissProt: Q07813 Mouse Unigene: 624291 Human Unigene: 19904 Mouse Unigene: 10668 Rat |
產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Bax (bsm-33279M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax ) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 久久久久久无码精品大片 | 精品999久久久一级毛片 | 免费一级婬片A片久久妖精97 | 91在线无码精品秘 在线-百度 | 成人无码AV一区二区 | 国产欧美一区二区精品性色超碰 | 欧一美一交一配一交一交一视频 | 四川野外少妇极品BBB | 国产传媒在线免费观看 | 国产三级精品三级在线 | 国产无码一区二区在线 | 强伦人妻一区二区三区视频18 | 人妻看护中文字幕 | 国产拍在线观看不卡 | 国产香蕉精品视频 | 99精品人人A片免费看 | 波多野结衣成人电影 | 中文字幕av在线观看 | 素人美女被操到高潮完整版在线观看 | 黄色Av一区二区三区 | 先锋影音资源av | 丰满岳妇乱一区二区三区 | 高清无码在线视频播放 | 日欧一片内射va在线影院 | www美女网站视频 | jk白丝护士一区二区三区 | 精品人妻无码一区二区三区 | 中国丰满熟女A片免费观 | AAA久久爽无码精品痴汉 | 精品人妻少妇嫩草Av | 成人做爰黄AA片免费看三区 | 日韩理论视频一本二本 | 26uuu精品一区二区三区 | 最骚少妇A片免费短视频 | 国产高清视频在线 | 91在线无码精品秘 在线-百度 | 日韩中文字幕在线观看 | 深夜福利网你懂的性爱视频自拍偷拍 | 99人妻人人揉人人澡人人 | 色情亂伦一级A片实拍 |