| 產(chǎn)品編號 | bsm-10807M |
| 英文名稱 | Human IL-6 Mouse mAb |
| 中文名稱 | 白介素6單克隆抗體 |
| 別 名 | Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmac |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 生長因子和激素 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 1C4 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human IL-6 protein: 30-212/212aa (C-6x His-Tag) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]. Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Subcellular Location: Secreted. Post-translational modifications: N- and O-glycosylated. DISEASE: Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Similarity: Belongs to the IL-6 superfamily. Gene ID: 3569 Database links: Entrez Gene: 3569 Human Entrez Gene: 16193 Mouse Omim: 147620 Human SwissProt: P05231 Human SwissProt: P08505 Mouse Unigene: 654458 Human Unigene: 1019 Mouse Unigene: 9873 Rat
生長因子和激素（ Growth Factor and Hormones） 白介素-6是一種多功能細胞因子，既可由淋巴細胞產(chǎn)生，也能由非淋巴細胞合成。在體內(nèi)免疫反應(yīng)調(diào)節(jié)、血細胞的增生、防御機制和急性期反應(yīng)中起重要作用。 IL6是一種糖蛋白，它既可誘導(dǎo)急性期時相關(guān)蛋白的產(chǎn)生，也是β細胞終末分化并分泌抗體的必需因子，在免疫調(diào)節(jié)中發(fā)揮重要作用，人的IL-6的前體有212個氨基酸。成熟形態(tài)為185個氨基酸。 白細胞介素6的分子量：21KD-28KD;二聚體結(jié)構(gòu)（多個亞型）：42KD-56KD |
| 產(chǎn)品圖片 |
HUVEC (H) cells were treated with or without LPS (0.5 μg/ml，24h) and Brefeldin A (300 ng/ml，20h) , 25 μg total protein per lane of cell lysates (see on figure) probed with human IL-6 monoclonal antibody, unconjugated (bsm-10807M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human IL-6 Monoclonal Antibody, Unconjugated(bsm-10807M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
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