| 產(chǎn)品編號 | bs-20815R |
| 英文名稱 | ADAM2 Rabbit pAb |
| 中文名稱 | 去整合素樣金屬蛋白酶2抗體 |
| 別 名 | A disintegrin and metalloproteinase domain 2; ADAM 2; ADAM metallopeptidase domain 2; ADAM2_MOUSE; AI323749; Cancer/testis antigen 15; CRYN1; CRYN2; CT15; Disintegrin and metalloproteinase domain-containing protein 2; Fertilin beta; Fertilin subunit beta; Ftnb; PH 30; PH 30b; PH-30; PH30; Ph30 beta; PH30-beta. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 細(xì)胞骨架 細(xì)胞外基質(zhì) 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63/80 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse ADAM2: 451-550/735 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ADAM-2, also known as PH-30?and Fertilin-? was first described as a sperm-egg fusion protein from guinea pig. A member of the metalloproteinase family containing disintigrin-like domains (ADAMs), the function of ADAM-2 is still poorly understood. Unlike ADAM-1, ADAM-2 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. Like the other ADAMs, ADAM-2 domain structure consists of a signal sequence followed by a propeptide domain, a metalloproteinase domain, a disintegrin domain cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. Three isoforms of ADAM-2 are reported to date, which differ in the beginning of the metalloproteinase domain and cysteine-rich domain. The sequences are coded from intronless genes. The longest ADAM-2 message encodes a protein of 735 amino acids, with a predicted mass of 82.5 kDa and a pI of 5.73. The 716 amino acid form of ADAM-2 has a deletion in the start of the metalloproteinase domain, relative to the longer form, and has a predicted mass of 80.2 kDa and pI of 5.75. The shorter form of 579 amino acids shared the deletion in the MP domain, and also has a deletion in the cystein-rich domain. Localized on the surface of sperm, the ADAM-2 isoforms are thought to form a heterodimer with ADAM-1 (fertilin-a), and facilitate sperm-egg fusion, although there is some controversy about the precise actions the proteins play. Integrin a6? on the egg surface is thought to dock with a QDECD motif in the disintegrin domain of ADAM-2, and there is some speculation that ADAM-1/ADAM-2 heterodimewr initiated ADAM-3 production on the cell surface. Function: Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. Subunit: Heterodimer with ADAM1/fertilin subunit alpha. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed specifically in testis. Post-translational modifications: The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. Database links: Entrez Gene: 2515 Human Omim: 601533 Human SwissProt: Q99965 Human Unigene: 177959 Human |
| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- ADAM2 (bs-20815R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63/80 kD
Observed band size: 63 kD
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