| 產(chǎn)品編號 | bs-21155R |
| 英文名稱 | RAP1GAP2 Rabbit pAb |
| 中文名稱 | RAP1GAP2蛋白抗體 |
| 別 名 | DKFZp686O238; GARNL4; GTPase activating RANGAP domain like 4; GTPase activating Rap/RanGAP domain like 4; GTPase activating Rap/RanGAP domain like protein 4; KIAA1039; RAP1 GTPase activating protein 2; RAP1GA3. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RAP1GAP2: 651-730/730 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a GTPase-activating protein that activates the small guanine-nucleotide-binding protein Rap1 in platelets. The protein interacts with synaptotagmin-like protein 1 and Rab27 and regulates secretion of dense granules from platelets at sites of endothelial damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Tissue Specificity: Cytoplasmic; perinuclear region. Similarity: Contains 1 RanBD1 domain. SWISS: Q684P5 Gene ID: 23108 Database links: Entrez Gene: 23108 Human Entrez Gene: 380711 Mouse SwissProt: Q684P5 Human SwissProt: Q5SVL6 Mouse Unigene: 499659 Human |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- RAP1GAP2 (bs-21155R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 76 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAP1GAP2) Polyclonal Antibody, Unconjugated (bs-21155R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
