| 產品編號 | bs-16653R |
| 英文名稱 | ING2 Rabbit pAb |
| 中文名稱 | 腫瘤生長抑制蛋白2抗體 |
| 別 名 | ING 1 like tumor suppressor protein; ING 1L; ING 2; ING1 like tumor suppressor protein; ING1L; ING1Lp; ING2; ING2_HUMAN; Inhibitor of growth 1 like; Inhibitor of growth 1 like protein; Inhibitor of growth 1-like protein; Inhibitor of growth family member 1 like; Inhibitor of growth family member 2; Inhibitor of growth protein 2; p32; p33 ING2; p33ING2. |
| 研究領域 | 腫瘤 細胞生物 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ING2: 151-250/280 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. [provided by RefSeq, Jul 2008] Function: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs). Subcellular Location: Nucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm. Tissue Specificity: Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues. Similarity: Belongs to the ING family. Contains 1 PHD-type zinc finger. SWISS: Q9H160 Gene ID: 3622 Database links: Entrez Gene: 3622 Human Entrez Gene: 69260 Mouse Omim: 604215 Human SwissProt: Q9H160 Human SwissProt: Q9ESK4 Mouse Unigene: 107153 Human Unigene: 430733 Mouse Unigene: 20482 Rat |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ING2 polyclonal antibody, unconjugated (bs-16653R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
