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CD200R Rabbit pAb (bs-10521R)  
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產(chǎn)品編號(hào) bs-10521R
英文名稱 CD200R Rabbit pAb
中文名稱 CD200受體抗體
別    名 CD 200R; CD200 cell surface glycoprotein receptor; CD200 receptor 1; Cd200r1; Cell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; Cell surface glycoprotein OX2 receptor; cell surface glycoprotein receptor CD200; CRTR 2; CRTR2; HCRTR2; MO2R1_HUMAN; MOX2 receptor; MOX2R; OX2R.  
Specific References  (2)     |     bs-10521R has been referenced in 2 publications.
[IF=2.49] Kuroi, Taiga. et al. Characterization of localized macrophages in bronchiolitis obliterans after allogeneic hematopoietic cell transplantation. 2021 Sep 07  IHC ;  human.  
[IF=2.48] Sun, Haoyu, et al. "CD200R, a co-inhibitory receptor on immune cells, predicts the prognosis of human hepatocellular carcinoma." Immunology Letters (2016).  IHC-P ;  Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  細(xì)胞膜受體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 33 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD200R: 201-300/325 <Extracellular>
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Function:
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.

Subcellular Location:
Secreted and Cell membrane.

Tissue Specificity:
Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.

Post-translational modifications:
The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.

Similarity:
Belongs to the CD200R family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q8TD46

Gene ID:
131450

Database links:

Entrez Gene: 131450 Human

Omim: 607546 Human

SwissProt: Q8TD46 Human

Unigene: 309158 Human



產(chǎn)品圖片
Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-CD200R (bs-10521R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-CD200R (bs-10521R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200R Polyclonal Antibody, Unconjugated(bs-10521R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Measured by its binding ability in a indirect ELISA. Immobilized Mycobacterium bovis protein (Cat. bs-4622P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-CD200R Antibody, the EC50 is 158 ng/mL.
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