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Sumo 3 Rabbit pAb (bs-15495R)  
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50ul/1180.00元
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200ul/2800.00元
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產(chǎn)品編號(hào) bs-15495R
英文名稱 Sumo 3 Rabbit pAb
中文名稱 泛素樣蛋白Sumo3抗體
別    名 Small ubiquitin-related modifier 3; SUMO-3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B; Smt3B; SUMO3_HUMAN.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  細(xì)胞凋亡  泛素  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Pig,Cow,Chicken,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 10.5 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Sumo3: 1-80/103 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).

Function:
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.

Subunit:
Covalently attached to a number of proteins (By similarity). Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I.

Subcellular Location:
Cytoplasm. Nucleus. Nucleus, PML body

Tissue Specificity:
Expressed predominantly in liver.

Post-translational modifications:
Polymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.

Similarity:
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.

SWISS:
P55854

Gene ID:
6612

Database links:

Entrez Gene: 6612 Human

Entrez Gene: 20610 Mouse

Entrez Gene: 499417 Rat

Omim: 602231 Human

SwissProt: P55854 Human

SwissProt: Q9Z172 Mouse

SwissProt: Q5XIF4 Rat

Unigene: 474005 Human

Unigene: 24433 Mouse

Unigene: 154914 Rat



產(chǎn)品圖片
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Sumo 3 Polyclonal Antibody, Unconjugated(bs-15495R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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