| 產(chǎn)品編號 | bs-12073R |
| 英文名稱 | P2Y8 Rabbit pAb |
| 中文名稱 | G蛋白偶聯(lián)嘌呤受體p2y8抗體 |
| 別 名 | P2RY8; P2RY8_HUMAN; P2Y purinoceptor 8; P2Y8; Purinergic receptor P2Y G protein coupled 8. |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 通道蛋白 細(xì)胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P2Y8: 131-230/359 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G-protein coupled receptors translate extracellular signals into intracellular signals (G-protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. P2RY8 (purinergic receptor P2Y, G-protein coupled, 8), also known as P2Y8, is a 359 amino acid multi-pass membrane protein that localizes to the cell membrane and belongs to the G protein-coupled receptor family. Expressed at low levels in lung, heart and kidney, P2RY8 may function as a receptor for purines that are coupled to G proteins and may also play a role in mental retardation. Function: Probable receptor for purines coupled to G-proteins. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Barely detectable in normal blood leukocytes. Weaker expression was seen in heart, kidney and lung. Not detected in brain. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q86VZ1 Gene ID: 286530 Database links: Entrez Gene: 286530 Human Omim: 300525 Human SwissProt: Q86VZ1 Human Unigene: 111377 Human |
| 產(chǎn)品圖片 |
Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti- P2Y8 (bs-12073R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 50 kD
Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (bs-12073R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (bs-12073R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
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