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Osteocalcin Rabbit pAb (bs-4917R)

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產(chǎn)品編號	bs-4917R
英文名稱	Osteocalcin Rabbit pAb
中文名稱	骨鈣蛋白/骨鈣素抗體
別名	OSTCN_HUMAN; BGLAP; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate protein; OC; OCN;
	Specific References (43) \| bs-4917R has been referenced in 43 publications. [IF=13.273] Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:131490 WB ; Rat. PubMed:10.1016/j.cej.2021.131490 [IF=11.322] Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554 IHC ; Rat. PubMed:10.1016/j.compositesb.2023.110554 [IF=10.652] Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084 IHC ; Rabbit. PubMed:10.1016/j.cej.2021.130084 [IF=10.319] Dan Li. et al. A HAase/NIR responsive surface on titanium implants for treating bacterial infection and improving osseointegration. J MATER SCI TECHNOL. 2022 Nov;: IF ; Rat. PubMed:10.1016/j.jmst.2022.09.029 [IF=9.77] Zheng-Zhao Liu . et al. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. 2020 Nov 04 IHC ; Mouse. PubMed:33143524 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC ; Rat. PubMed:10.1016/j.apmt.2020.100673 [IF=8.31] Li, Long, et al. "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." Biomaterials (2014). IHC-P ; Rat. PubMed:25453952 [IF=8.025] Yixuan Li. et al. Minimally invasive bone augmentation through subperiosteal injectable hydroxylapatite/laponite/alginate nanocomposite hydrogels. INT J BIOL MACROMOL. 2023 Mar;231:123232 IHC ; Rat. PubMed:36681217 [IF=7.666] Maximilian Koblenzer. et al. Physiological Mineralization during In Vitro Osteogenesis in a Biomimetic Spheroid Culture Model. CELLS-BASEL. 2022 Jan;11(17):2702 IHC ; Mouse. PubMed:36078105 [IF=7.666] Katarzyna Klimek. et al. Could Curdlan/Whey Protein Isolate/Hydroxyapatite Biomaterials Be Considered as Promising Bone Scaffolds?—Fabrication, Characterization, and Evaluation of Cytocompatibility towards Osteoblast Cells In Vitro. CELLS-BASEL. 2022 Jan;11(20):3251 IF ; Human. PubMed:36291119 [IF=7.59] Zhenyin Chen. et al. Dynamic stiffening collagen-coated substrate enhances osteogenic differentiation of mesenchymal stem cells through integrin α2β1. BIOMATER SCI-UK. 2023 May;: IF ; Rat. PubMed:37233200 [IF=7.31] Guangyue Li. et al. Irisin Promotes Osteogenesis by Modulating Oxidative Stress and Mitophagy through SIRT3 Signaling under Diabetic Conditions. OXID MED CELL LONGEV. 2022 Oct 10;2022:3319056 IHC ; Rat. PubMed:36262283 [IF=6.895] Liu M et al. Luminescent Net-like Inorganic Scaffolds with Europium Doped Hydroxyapatite for Enhanced Bone ReconstructionNanoscale.2021 Jan 6. IF ; Human. PubMed:33404034 [IF=6.344] Zheng, Han. et al. miR-140-3p enhanced the osteo/odontogenic differentiation of DPSCs via inhibiting KMT5B under hypoxia condition. Int J Oral Sci. 2021 Dec;13(1):1-10 WB ; Human. PubMed:34876565 [IF=6.208] Samantha Ketelyn Silva. et al. Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation. INT J MOL SCI. 2022 Jan;23(12):6503 IHC ; Rat. PubMed:35742948 [IF=5.988] Kou Yuying. et al. Eldecalcitol prevented OVX-induced osteoporosis through inhibiting BMSCs senescence by regulating the SIRT1-Nrf2 signal. FRONT PHARMACOL. 2023 Mar;14:504 WB ; Rat. PubMed:36937895 [IF=5.88] F?rster Y et al. The influence of different artificial extracellular matrix implant coatings on the regeneration of a critical size femur defect in rats. Mater Sci Eng C Mater Biol Appl . 2020 Nov;116:111157. IHF-P ; Rat. PubMed:32806270 [IF=5.88] Qiuping Leng. et al. Demineralized bone matrix scaffold modified with mRNA derived from osteogenically pre-differentiated MSCs improves bone repair. Mat Sci Eng C-Mater. 2021 Feb;119:111601 IHC ; Rat. PubMed:33321645 [IF=5.854] Yi Liu. et al. Modification of COL1A1 in Autologous Adipose Tissue-Derived Progenitor Cells Rescues the Bone Phenotype in a Mouse Model of Osteogenesis Imperfecta. 2021 May 18 IF ; Mouse. PubMed:33950576 [IF=5.711] Zhu H et al. Direct reprogramming of mouse fibroblasts into functional osteoblasts. J Bone Miner Res. 2019 Dec 2. ICF ; Mouse. PubMed:31793059 [IF=5.157] Qingnan Yan. et al. Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling. J Biol Chem. 2022 Jan;:101639 IF ; Mouse. PubMed:35090892 [IF=5.051] Du Zhi. et al. Circulating exosomal circRNA_0063476 impairs expression of markers of bone growth via the miR-518c-3p/DDX6 axis in ISS. ENDOCRINOLOGY. 2022 Aug;: IHC ; Rat. PubMed:35974445 [IF=4.652] Jiang et al. Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro. (2015) Cell.Physiol.Bioche. 36:2327-39 ICC ; Rat. PubMed:26279437 [IF=4.545] Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235. IHC ; Mouse. PubMed:32454289 [IF=4.15] Ding, Shan, et al. "A nano-micro alternating multilayer scaffold loading with rBMSCs and BMP-2 for bone tissue engineering." Colloids and Surfaces B: Biointerfaces (2015). IHC-P ; Rat. PubMed:26119373 [IF=4.122] ?brahim Halilullah Erbay. et al. Bioengineering bone-on-a-chip model harnessing osteoblastic and osteoclastic resolution. ADV ENG MATER. 2022 Dec;: IF ; Mouse. PubMed:10.1002/adem.202201063 [IF=3.989] Liu G et al. Suppressing?MicroRNA-30b?by?Estrogen?Promotes?Osteogenesis?in?Bone?Marrow?MesenchymalStem?Cells. Stem Cells Int. 2019 Apr 4;2019:7547506. WB ; Rat. PubMed:31089333 [IF=3.869] Zhang Chen. et al. The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla. Stem Cells Int. 2020;2020:8881021 WB ; Humann. PubMed:33082788 [IF=3.448] Xiao Y et al. Controlled delivery of recombinant human bone morphogenetic protein-2 by using glucose-sensitive core–shell nanofibers to repair the mandible defects in diabetic rats. J. Mater. Chem. B, 2019,7, 4347-4360. ICF ; Human. PubMed:doi:10.1039/C9TB00613C [IF=3.328] Nan Wang. et al. Histological analysis of pulp mineralisation after severe intrusive luxation of immature molars in rats. DENT TRAUMATOL. 2023 Feb;: IHC ; Rat. PubMed:36807827
研究領(lǐng)域	細胞生物信號轉(zhuǎn)導(dǎo) 干細胞細胞外基質(zhì)
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	11 kDa
檢測分子量
細胞定位	分泌型蛋白
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Osteocalcin: 21-100/100
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Subcellular Location: Secreted. Post-translational modifications: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. Similarity: Belongs to the osteocalcin/matrix Gla protein family. Contains 1 Gla (gamma-carboxy-glutamate) domain. SWISS: P02818 Gene ID: 632 Database links: Entrez Gene: 281646 Cow Entrez Gene: 632 Human Entrez Gene: 397530 Pig Entrez Gene: 25295 Rat Omim: 112260 Human SwissProt: P02820 Cow SwissProt: P02818 Human SwissProt: Q8HYY9 Pig SwissProt: P04640 Rat Unigene: 654541 Human Unigene: 9722 Rat 骨鈣素又稱骨γ-羧谷氨酸包含蛋白
產(chǎn)品圖片	Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human MG63 cell lysates Lane 3: Human A549 cell lysates Lane 4: Human U-2 OS cell lysates Primary: Anti-Osteocalcin (bs-4917R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei stainin

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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