| 產(chǎn)品編號 | bs-6461R |
| 英文名稱 | NFATC4 Rabbit pAb |
| 中文名稱 | T細(xì)胞激活核轉(zhuǎn)錄因子4抗體 |
| 別 名 | NF-ATc4; NFATC4 cytoplasmic 4; NF ATc4; NF-AT3; NF-ATc4; NFAC4_HUMAN; NFAT3; NFATc4; Nuclear factor of activated T cells cytoplasmic 4; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4; Nuclear factor of activated T-cells; nuclear factor of activated T-cells cytoplasmic 4; Nuclear factor of activated T-cells cytoplasmic calcineurin-dependent 4; T cell transcription factor NFAT3; T-cell transcription factor NFAT3. |
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Specific References (6) | bs-6461R has been referenced in 6 publications.
[IF=8.063] Zhang Y et al. ASIC1a induces synovial inflammation via the Ca2+/NFATc3/RANTES pathway. Theranostics. 2020 Jan 1;10(1):247-264. IHF-P ; Rat.
[IF=5.039] Zhang B et al. miR-137 and its target T-type CaV 3.1 channel modulate dedifferentiation and proliferation of cerebrovascular smooth muscle cells in simulated microgravity rats by regulating calcineurin/NFAT pathway. Cell Proliferation. 2020;00:e12774. WB ; Rat.
[IF=3.263] Li L et al. Sacubitril/valsartan attenuates atrial electrical and structural remodelling in a rabbit model of atrial fibrillation. Eur J Pharmacol
. 2020 Aug 15;881:173120. IHC,WB ; rabbit.
[IF=2.81] Xie, Sheng, et al. "Chronic intermittent hypoxia induces cardiac hypertrophy by impairing autophagy through the adenosine 5′-monophosphate-activated protein kinase pathway." Archives of Biochemistry and Biophysics (2016). WB ; Rat.
[IF=2.373] Miguel H. Santamaría. et al. Trypanosoma cruzi down-regulates adiponectin expression in mouse adipocytes via the NFAT signaling pathway. Microbes Infect. 2021 Jan;23:104757 WB ; Mouse.
[IF=2.107] Su Q et al. Involvement of calcineurin/NFATc4 pathway in a single-prolonged stress-based rat model of post-traumatic stress disorder. Mol Biol Rep. 2019 Sep 4. WB ; Rat.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 淋巴細(xì)胞 t-淋巴細(xì)胞 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 95 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NF-ATc4: 475-525/902 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The product of this gene is a member of the nuclearfactors of activated T cells DNA-binding transcription complex.This complex consists of at least two components: a preexistingcytosolic component that translocates to the nucleus upon T cellreceptor (TCR) stimulation and an inducible nuclear component.Other members of this family of nuclear factors of activated Tcells also participate in the formation of this complex. Theproduct of this gene plays a role in the inducible expression ofcytokine genes in T cells, especially in the induction of the IL-2and IL-4. Alternatively spliced transcript variants encodingdifferent isoforms have been noted for this gene. Function: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons. Subunit: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with CREBBP, GATA4, IRAK1, MAPK8, MAPK9 and RPS6KA3. Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. Tissue Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus. Post-translational modifications: Phosphorylated by NFATC-kinases; dephosphorylated bycalcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1,MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and onSer-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B. Ubiquitinated, leading to its degradation by the proteasomeand reduced transcriptional activity. Ubiquitination and reductionin transcriptional activity can be further facilitated throughGSK3B-dependent phosphorylation. Polyubiquitin linkage is mainlythrough 'Lys-48'. Similarity: Contains 1 IPT/TIG domain. Contains 1 RHD (Rel-like) domain. SWISS: Q14934 Gene ID: 4776 Database links: Entrez Gene: 4776 Human Entrez Gene: 73181 Mouse Omim: 602699 Human SwissProt: Q14934 Human SwissProt: Q8K120 Mouse Unigene: 77810 Human Unigene: 27908 Mouse Unigene: 23727 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFATC4) Polyclonal Antibody, Unconjugated (bs-6461R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFATC4) Polyclonal Antibody, Unconjugated (bs-6461R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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