| 產(chǎn)品編號 | bs-0345G-HRP |
| 英文名稱 | Goat Anti-Human IgM antibody |
| 中文名稱 | 辣根過氧化物酶標記的羊抗人IgM |
| 別 名 | Goat Anti-Human IgM (HRP); Immunoglobulin M; 羊抗人-IgM; |
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Specific References (3) | bs-0345G-HRP has been referenced in 3 publications.
[IF=22.553] Ni?L et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity 2020 06 16;526(6) ELISA ; Human.
[IF=5.085] Ling Ni. et al. Impaired Cellular Immunity to SARS-CoV-2 in Severe COVID-19 Patients. Front Immunol. 2021; 12: 603563 ELISA ; Human.
[IF=2.3] Tian, Lifang, et al. "Autoimmune and inflammatory responses in Kashin?CBeck disease compared with rheumatoid arthritis and osteoarthritis."Human Immunology 72.10 (2011): 812-816.. ELISA ; Human.
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| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-10000,IHC-P=1:100-500,IHC-F=1:100-1000,ELISA=1:1000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 2.0 mg/ml |
| 免 疫 原 | Native Human IgM |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human IgM(bs-0345P)
Primary: Anti-Human IgM / HRP (bs-0345G-HRP) at 1/20000 dilution
Predicted band size: kDa
Observed band size: kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
