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[IF=5.26] Meng, Guoliang, et al. "Hydrogen sulfide pretreatment improves mitochondrial function in myocardial hypertrophy via a SIRT3 dependent manner." British Journal of Pharmacology (2017).  WB ;  Mouse.  

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[IF=4.315] Ma, Dufang. et al. Astragalus polysaccharide prevents heart failure-induced cachexia by alleviating excessive adipose expenditure in white and brown adipose tissue. LIPIDS HEALTH DIS. 2023 Dec;22(1):1-16  WB ;  Rat.  

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[IF=3.776] Tingting Zhao. et al. Yishen Huashi granule modulated lipid metabolism in diabetic nephropathy via PI3K/AKT/mTOR signaling pathways.. HELIYON. 2023 Mar;9(3):e14171-e14171  WB ;  Human.  

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[IF=3.189] Tingting Li. et al. Cold exposure induces browning of bovine subcutaneous white fat in vivo and in vitro. J THERM BIOL. 2023 Feb;112:103446  WB ;  Bovine.  

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[IF=2.952] Ji Hye Hwang. et al. Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway. MOL MED REP. Mol Med Rep. 2022 Jun;25(6):1-10  WB ;  Mouse.  

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Sample: Lane 1: Molt-4 (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: Hela (Human) Cell Lysate at 30 ug Lane 5: Jurkat (Human) Cell Lysate at 30 ug Lane 6: HL60 (Human) Cell Lysate at 30 ug Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90/113 kD Observed band size: 113 kD

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (bs-7535R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PGC1 alpha+beta Polyclonal Antibody, Unconjugated(bs-7535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-PGC1 alpha+beta antibody (bs-7535R) Dilution:1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.