| 產(chǎn)品編號 | bs-6587R |
| 英文名稱 | APEX2 Rabbit pAb |
| 中文名稱 | 嘌呤嘧啶核酸內(nèi)切酶2抗體 |
| 別 名 | AP endonuclease 2; AP endonuclease XTH2; APE 2; APE2; APEX 2; APEX L2; APEX nuclease(apurinic/apyrimidinic endonuclease) 2; APEX Nuclease 2; APEX nuclease like 2; APEXL 2; APEXL2; Apurinic apyrimidinic endonuclease 2; Apurinic/apyrimidinic endonuclease 2; Apurinic/apyrimidinic endonuclease like 2; C430040P13Rik; DNA(apurinic or apyrimidinic site) lyase 2; XTH 2; XTH2; APEX2_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 染色質(zhì)和核信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APEX2: 171-270/518 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER). Function: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Subunit: Interacts with PCNA; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Subcellular Location: Nucleus. Cytoplasm. Mitochondrion (Probable). Note=Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. Tissue Specificity: Highly expressed in brain and kidney. Weakly expressed in the fetal brain. Similarity: Belongs to the DNA repair enzymes AP/ExoA family. SWISS: Q9UBZ4 Database links: Entrez Gene: Human Entrez Gene: 77622 Mouse Omim: 300773 Human SwissProt: Q9UBZ4 Human SwissProt: Q68G58 Mouse Unigene: 659558 Human Unigene: 440275 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lymph node(Mouse) Lysate at 40 ug
Primary: Anti- APEX2 (bs-6587R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human U87MG cell lysates
Lane 5: Human A431 cell lysates
Lane 6: Human MCF-7 cell lysates
Lane 7: Human HeLa cell lysates
Lane 8: Human LOVO cell lysates
Primary: Anti-APEX2 (bs-6587R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
