產(chǎn)品編號(hào) | bs-6506R |
英文名稱 | ABI3BP Rabbit pAb |
中文名稱 | ABI基因家族成員3結(jié)合蛋白抗體 |
別 名 | ABI gene family member 3(NESH) binding protein; ABI gene family member 3 binding protein; ABI3BP; Nesh binding protein; Nesh BP; NESHBP; Target of Nesh SH3; TARSH; TARSH_HUMAN. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 腫瘤細(xì)胞生物標(biāo)志物 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 116 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ABI3BP: 931-1075/1075 |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
ABI3BP (ABI gene family member 3-binding protein) contains 2 fibronectin type-III domains and is thought to interact with ABI3. It is expressed in brain, heart, lung, liver, pancreas, kidney and placenta. ABI3BP expression is dramatically reduced in human lung cancer cell lines and primary lung tumor, while it is predominantly expressed in normal conditions, suggesting that it is involved in prevention of tumorigenesis. Subunit: Probably interacts with ABI3. Subcellular Location: Secreted. Tissue Specificity: Expressed in brain, heart, lung, liver, pancreas kidney and placenta. Similarity: Contains 2 fibronectin type-III domains. SWISS: Q7Z7G0 Gene ID: 25890 Database links: Entrez Gene: 25890 Human Omim: 606279 Human SwissProt: Q7Z7G0 Human Unigene: 477015 Human |
產(chǎn)品圖片 |
Sample:
Placenta (Mouse) Lysate at 40 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- ABI3BP (bs-6506R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ABI3BP Polyclonal Antibody, Unconjugated(bs-6506R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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