產(chǎn)品編號 | bs-4003R |
英文名稱 | phospho-eIF4G (ser1185) Rabbit pAb |
中文名稱 | 磷酸化真核翻譯起始因子4G抗體 |
別 名 | eIF4G(phospho-Ser1185); eIF4G(phospho-S1185); p-eIF4G(Ser1185); p-eIF4G(S1185); eIF 4 gamma 1; eIF 4G 1; eIF 4G1; EIF4 gamma; EIF4F; EIF4G; EIF4GI; Eukaryotic translation initiation factor 4 gamma 1; p220; DKFZp686A1451; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; EIF4G-I; IF4G1_HUMAN. |
![]() |
Specific References (1) | bs-4003R has been referenced in 1 publications.
[IF=5.923] Emma Martínez-Alonso. et al. Phosphorylation of Eukaryotic Initiation Factor 4G1 (eIF4G1) at Ser1147 Is Specific for eIF4G1 Bound to eIF4E in Delayed Neuronal Death after Ischemia. Int J Mol Sci. 2022 Jan;23(3):1830 WB ; Rat.
|
產(chǎn)品類型 | 磷酸化抗體 |
研究領(lǐng)域 | 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 176 kDa |
檢測分子量 | |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human eIF4G around the phosphorylation site of ser1185: KR(p-S)FS |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Summary: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq]. Function: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Subunit: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with CIRBP and MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis. Interacts with RBM4. Post-translational modifications: Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription. DISEASE: Defects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Similarity: Belongs to the eIF4G family. Contains 1 MI domain. Contains 1 MIF4G domain. Contains 1 W2 domain. SWISS: Q04637 Gene ID: 1981 Database links: Entrez Gene: 1981 Human Entrez Gene: 208643 Mouse Omim: 600495 Human SwissProt: Q04637 Human SwissProt: Q6NZJ6 Mouse Unigene: 433750 Human Unigene: 260256 Mouse Unigene: 101803 Rat |
1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 国产白丝视频唐伯虎 | www.亚洲555久久久 | 一级亚洲看片鲁在线观看 | 久久精品在线视频网站在线视频 | 一级黄色日本A级片试看2分 | 中文在线永久免费观看 | 明里 无码一区二区三区 | 99成人兔费视频在线播放 | 久久96国产精品久久99软件 | 国产精品揄拍一区二区 | 成人全黄A片免费网站 | 久久久久91精品視頻亞洲一區二區三區 | 搡BBBB搡BBB搡18| 免费无遮挡无码永久在线观看视频 | 国产又粗又黄又爽又硬的免费视频 | 夜阁视频动态图片在线观看 | 91蜜桃传媒麻豆中文字幕 | 亚洲熟妇少妇熟女A片百度知道 | 少妇喷白浆高清AV含羞草 | 91精品国产蜜臀色欲 | 亚洲人无码成www久久 | 国产一级a毛一级a看免费视频乱 | 经典hs电影在线 | 久久国产精品高潮一级毛片 | 黑人大属又大又粗又长 | 久久欧美国产伦子伦精品 | 人人妻人人澡人人爽人人精品图片 | 久久久亚洲欧洲日产国码αv、、 | 少妇一级婬片50分钟 | 黄色成人视频在线观看 | 国产美女美乳奶头粉嫩在线观看 | 麻豆视频破解在线无限观看 | 中国女人内谢69xxx | 黄片在线免费观看视频 | 麻豆乱码国产一区二区三区 | 欧美在线无码精品秘 蜜桃 国产精品一级无码毛片视频 | 国产欧美精品啪啪网站 | 人妻av一区二区三区 | 欧美交换乱婬A片免费 | 无码人妻精品一区二区蜜桃网站 |