| 產(chǎn)品編號 | bs-3681R |
| 英文名稱 | Adenylate cyclase 1 Rabbit pAb |
| 中文名稱 | 腺苷酸環(huán)化酶1抗體 |
| 別 名 | 3'5' cyclic AMP synthetase; ADCY1; adenyl cyclase; adenylate cyclase type I; ATP pyrophosphate lyase; Brain adenylate cyclase 1; Ca(2+)/calmodulin activated adenylyl cyclase; EC 4.6.1.1; ADCY1_HUMAN; Adenylate cyclase type 1; ATP pyrophosphate-lyase 1; Ad |
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Specific References (1) | bs-3681R has been referenced in 1 publications.
[IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292 WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 123 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human adenylate cyclase type I: 751-850/1119 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The membrane bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein coupled receptors. Eight human AC isoforms (AC-1 through AC-4) have been identified up to now and genes for at least nine adenylate cyclase (AC-1-AC-9) have been cloned characterized and sequenced.
This is a calmodulin-sensitive adenylyl cyclase which may be involved in regulatory processes in the central nervous system. It may play a role in neuronal plasticity, memory acquisition and learning and is expressed predominantly in the brain, retina and adrenal medulla. Function: This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Brain, retina and adrenal medulla. Similarity: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains. SWISS: Q08828 Gene ID: 107 Database links: Entrez Gene: 107 Human Entrez Gene: 432530 Mouse Omim: 103072 Human SwissProt: Q08828 Human SwissProt: O88444 Mouse Unigene: 192215 Human Unigene: 259733 Mouse 腺苷酸環(huán)化酶(adenylate cyclase, AC) 是G蛋白偶聯(lián)受體家族之一，是膜整合蛋白,它的氨基端和羧基端都朝向細(xì)胞質(zhì)。AC在膜的細(xì)胞質(zhì)面有兩個催化結(jié)構(gòu)域,還有兩個膜整合區(qū),每個膜整合區(qū)分別有6個跨膜的α螺旋。哺乳動物中已發(fā)現(xiàn)6個腺苷酸環(huán)化酶異構(gòu)體。由于AC能夠?qū)TP轉(zhuǎn)變成cAMP,引起細(xì)胞的信號應(yīng)答,因此,腺苷酸環(huán)化酶是G蛋白偶聯(lián)系統(tǒng)中的效應(yīng)物。 |
| 產(chǎn)品圖片 |
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- ADCY1 (bs-3681R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123kD
Observed band size: 123kD
Sample:
MCF-7 Cell(Human)Lysate at 30 ug
Primary: Anti- ADCY1 (bs-3681R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123kD
Observed band size: 123kD
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylate cyclase 1) Polyclonal Antibody, Unconjugated (bs-3681R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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