| 產(chǎn)品編號 | bs-3673R |
| 英文名稱 | DIO2 Rabbit pAb |
| 中文名稱 | 脫碘酶2抗體 |
| 別 名 | 5DII; D2; Deiodinase, iodothyronine, type II; DIOII; ITDI2; SelY; Thyroxine deiodinase, type II; TXDI2; Type 2 DI; Type II 5' deiodinase; Type II iodothyronine deiodinase. |
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Specific References (2) | bs-3673R has been referenced in 2 publications.
[IF=6.551] Mei Ha. et al. PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. Sci Total Environ. 2021 Feb;757:143727 WB ; Rat.
[IF=3.177] Enoch Appiah Adu-Gyamfi. et al. Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage. Placenta. 2021 Aug;111:54 WB,IF ; Human.
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| 研究領(lǐng)域 | 免疫學(xué) 生長因子和激素 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DIO2: 101-200/273 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms. Function: Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Subunit: Interacts with USP20 and USP33. Interacts with MARCH6. Subcellular Location: Membrane; Single-pass membrane protein (Potential). Tissue Specificity: Heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. Post-translational modifications: Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33. Similarity: Belongs to the iodothyronine deiodinase family. SWISS: Q92813 Gene ID: 1734 Database links: Entrez Gene: 1734 Human Entrez Gene: 13371 Mouse Omim: 601413 Human SwissProt: Q92813 Human SwissProt: Q9Z1Y9 Mouse Unigene: 202354 Human Unigene: 21389 Mouse Unigene: 408815 Mouse Unigene: 88380 Rat 脫碘酶2泛稱Ⅱ型碘甲腺氨酸脫碘酶脫碘，是調(diào)節(jié)甲狀腺激素生物活性的特別方式。脫碘酶可加速T4轉(zhuǎn)化為T3，引起甲狀腺激素及生長激素分泌減少，影響一些機(jī)體生物學(xué)的改變。 DIO2主要表達(dá)在中樞神經(jīng)系統(tǒng)、垂體前葉腺體、棕色脂肪組織和胎盤內(nèi)。其他組織中也有一定量的分布：心肌和骨胳肌、肝、腎、中樞神經(jīng)系統(tǒng)、 腦，皮膚，胎盤等。 |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-DIO2 (bs-3673R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (DIO2) Polyclonal Antibody, Unconjugated (bs-3673R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Thyroid tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (DIO2) Polyclonal Antibody, Unconjugated (bs-3673R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (bs-3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (bs-3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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