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KEAP1 Rabbit pAb (bs-3648R)

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產(chǎn)品編號	bs-3648R
英文名稱	KEAP1 Rabbit pAb
中文名稱	胞質(zhì)接頭蛋白Keap1抗體
別名	Cytosolic inhibitor of Nrf2; INrf 2; INrf2; Keap 1; KEAP1; KEAP1_HUMAN; Kelch like ECH associated protein 1; Kelch like protein 19; Kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132; KLHL 19; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454.
	Specific References (26) \| bs-3648R has been referenced in 26 publications. [IF=7.675] Xiaoxiao Zou. et al. Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice. ANTIOXIDANTS-BASEL. 2023 Mar;12(3):712 WB ; Mouse. PubMed:36978962 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965 WB ; Duck. PubMed:34775344 [IF=5.81] Xinyue Chang. et al. The Protective Effect of Trichilia catigua A. Juss. on DEHP-Induced Reproductive System Damage in Male Mice. Front Pharmacol. 2022; 13: 832789 IHC ; Mouse. PubMed:35185586 [IF=5.552] Huang, Jianjia. et al. Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways. J ZHEJIANG UNIV-SC B. 2023 Feb;24(2):157-171 WB ; Dog. PubMed:36751701 [IF=5.195] Yiyun Qian. et al. Seeds of Ginkgo biloba L. inhibit oxidative stress and inflammation induced by cigarette smoke in COPD rats through the Nrf2 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115758 WB ; Rat. PubMed:36167232 [IF=4.868] Chen W et al. Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice. Oxidative Medicine and Cellular Longevity, 2019, 1–17. WB ; Mouse. PubMed:doi:10.1155/2019/7419249 [IF=4.545] Yingran Liang. et al. Crocin ameliorates arsenic trioxide?induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713 WB ; Rat. PubMed:32920515 [IF=4.529] Shikuo Rong. et al. Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-κB and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia. MEDIAT INFLAMM. 2022 Dec 06;2022:5184721 WB ; Mouse. PubMed:36523959 [IF=4.389] Junrong Zhang. et al. Neuroprotective effects of maize tetrapeptide-anchored gold nanoparticles in Alzheimer’s disease. Colloid Surface B. 2021 Apr;200:111584 WB ; Mouse. PubMed:33508658 [IF=4.36] Song Yang. et al. Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo. J Ethnopharmacol. 2021 Oct;:114739 WB ; Mouse. PubMed:34648903 [IF=4.19] Su, Chuanyang, et al. "Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation, but not Keap1-Cullin3 complex dissociation." Chemical Research in Toxicology (2015). WB ; Human. PubMed:25742418 [IF=4.109] Yingrong Ye. et al. Investigations into ferroptosis in methylmercury-induced acute kidney injury in mice. ENVIRON TOXICOL. 2023 Mar;: WB ; Mouse. PubMed:36880449 [IF=4.105] Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1α proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963 WB ; Human. PubMed:33444648 [IF=3.905] Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025 IF ; Human. PubMed:35026282 [IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653 WB ; Human. PubMed:28974930 [IF=3.743] Jin W et al. Tannic acid ameliorates arsenic trioxide-induced nephrotoxicity, contribution of NF-κB and Nrf2 pathways. Biomed Pharmacother. 2020 Jun;126:110047. WB ; rat. PubMed:32146384 [IF=3.639] Huiling Mao. et al. Leucine protects bovine intestinal epithelial cells from hydrogen peroxide-induced apoptosis by alleviating oxidative damage. J SCI FOOD AGR. 2022 May 05 WB ; Bovine. PubMed:35437753 [IF=3.58] Su, Chuanyang, et al. "The electrophilic character of quinones is essential for the suppression of Bach1." Toxicology (2017). PubMed:28645578 [IF=3.571] Zhao Y et al. Lycopene prevents DEHP-induced Leydig cell damage with the Nrf2 antioxidant signaling pathway in mice. J Agric Food Chem. 2019 Dec 8. WB ; Mouse. PubMed:31814398 [IF=3.23] Chen, Ya-Hui, et al. "Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia." PLOS ONE11.4 (2016): e0154210. WB, IHC-P ; Rabbit. PubMed:27124120 [IF=3.205] Meng et al. Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1. (2018) J.Anim.Sci.Biotechnol. 9:34 WB ; Sow. PubMed:29713468 [IF=3.11] Su, Chuanyang, et al. "Neohesperidin dihydrochalcone against CCl4-induced hepatic injury through different mechanisms The implication of free radical scavenging and Nrf2 activation." Journal of Agricultural and Food Chemistry(2015). WB ; Human. PubMed:25978654 [IF=2.639] Jin Houet al. Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway. Biol Trace Elem Res . 2021 Mar;199(3):1062-1073. WB ; mouse. PubMed:32666434 [IF=1.892] Kaiqiang Fu. et al. Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway. Res Vet Sci. 2021 May;136:220 WB ; Bovine. PubMed:33689878 [IF=1.719] Kacar S et al. Effect?of?acrylamide?on?BEAS-2B?normal?human?lung?cells:?Cytotoxic,?oxidative,?apoptotic?and?morphometric?analysis. Acta Histochem. 2019 Jul;121(5):595-603. ICC ; Human. PubMed:31109687 [IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27 WB ; Bovine. PubMed:33866621
研究領域	腫瘤細胞生物免疫學信號轉(zhuǎn)導細胞凋亡轉(zhuǎn)錄調(diào)節(jié)因子激酶和磷酸酶
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog)
產(chǎn)品應用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	69 kDa
檢測分子量
細胞定位	細胞核細胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human KEAP1: 65-160/624
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. Subunit: Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Subcellular Location: Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus. Tissue Specificity: Broadly expressed, with highest levels in skeletal muscle. Similarity: Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. SWISS: Q14145 Gene ID: 9817 Database links: Entrez Gene: 9817 Human Entrez Gene: 50868 Mouse Entrez Gene: 100136900 Pig Entrez Gene: 117519 Rat Omim: 606016 Human SwissProt: Q14145 Human SwissProt: Q9Z2X8 Mouse SwissProt: Q684M4 Pig SwissProt: P57790 Rat Unigene: 465870 Human Unigene: 248266 Mouse Unigene: 23467 Rat Keap1是細胞抗氧化反應的重要調(diào)節(jié)蛋白，Keap1與Nrf2/ARE構(gòu)成的信號通路在抗腫瘤、抗應激、調(diào)節(jié)GSH合成、抗凋亡、抗炎反應、神經(jīng)保護等方面發(fā)揮著廣泛的細胞保護作用。
產(chǎn)品圖片	This image was generously provided by Yang Song, Ph.D. at Southwest University in Chong Qing, China. HepG2 cells were incubated with Rabbit Anti-KEAP1 Polyclonal Antibody (bs-3648R) at 4°C overnight and then mixed with Protein A agarose beads at 4°C for 3 Sample: A673(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-KEAP1 (bs-3648R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Ant Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation wi Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; An

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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