產(chǎn)品編號 | bs-2540R |
英文名稱 | Rabbit Anti-GLUT12 antibody |
中文名稱 | 葡萄糖轉(zhuǎn)運(yùn)蛋白12抗體 |
別 名 | GLUT-12; GTR12_HUMAN; Glucose transporter type 12; Glucose transporter type 12; GLUT 12; GLUT8; SLC2A12; Solute carrier family 2(facilitated glucose transporter) member 12; Solute carrier family 2 facilitated glucose transporter member 12; Solute carrier family 2 member 12. |
![]() |
Specific References (8) | bs-2540R has been referenced in 8 publications.
|
研究領(lǐng)域 | 免疫學(xué) 轉(zhuǎn)運(yùn)蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,GuineaPig,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 68 kDa |
檢測分子量 | |
細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GLUT12: 251-350/617 <Extracellular> |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
GLUT12 belongs to a family of transporters that catalyze the uptake of sugars through facilitated diffusion. Thirteen different types of glucose/fructose transport carrier proteins designated as Glut 1-13 facilitate glucose/fructose transport across the cell membrane. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. Function: Facilitative glucose transporter. Subcellular Location: Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region. Note=Localizes primarily perinuclear region in the absence of insulin. Tissue Specificity: Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney. Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. SWISS: Q8TD20 Gene ID: 154091 Database links: Entrez Gene: 154091?/span>Human Entrez Gene: 353169?/span>Mouse Entrez Gene: 308028?/span>Rat Omim: 610372?/span>Human SwissProt: Q8TD20?/span>Human SwissProt: Q8BFW9?/span>Mouse Unigene: 486508?/span>Human Unigene: 473705?/span>Mouse |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with GLUT12 polyclonal antibody, unconjugated (bs-2540R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut12 Polyclonal Antibody, Unconjugated(bs-2540R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded Human Gastric fundus adenocarcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
A-549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (bs-2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (bs-2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
The MCF-7(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-GLUT12 antibody (bs-2540R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF647 (bs-40295G-BF647): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
|
| 亚洲中文字幕一区 | 无码人妻aⅴ一区二区三区有奶水 | 91嫖妓站街按摩店老熟女 | 日韩内射美女人妻一区二区三区 | 国产丰满又爽 又黄 | 国产真实乱人偷精品视频 | 国产精品国产一级A片精品乌克兰 | 伊人婷婷色五月色婷婷区 | 久久国产精品波多野结衣AV孕妇 | 国产午夜精品理论片A级探花 | 午夜精品久久久久久久99老熟妇 | 国产农村乱来免费A片 | 午夜精品秘 一区二区三区 中文字幕av久久爽一区 | 特级西西444www无码视频免费看 | 99人妻少妇无码αⅤ二区下载 | X9X9X9搡BBBB搡BBB 国产毛片精品一区二区色欲黄A片 | 亚洲淫色人妻无码在线 | 91无码人妻精品一区二区三区四 | 日韩人妻丰满无码区A片 | 午夜成人电影在线观看 | 少妇性BBB搡BBB爽爽爽动漫 | 国产裸体视频BBBBB | 看美女操皮黄色毛片 | 国产精品污www在线观看 | 玩弄奶水人妻无码A∨在线 成人欧美精品久久久久影院 | 特级毛片A片久久久久久 | 潘金莲一级婬片AAA 91丨九色丨熟女高潮 | 色综合天天综合网国产成人网 | 午夜视频网站在线观看 | 我看一级毛片一级强奸片一级强暴片毛片 | 欧美精品-老牛影视内射 | 精品无码人妻一区二区免费 | 熟妇探花一区二区三区 | 美女自慰久久久www 美女视频网站直接进入 | 香蕉网在线免费播放视频 | 国产精品无码久久久久 | 一级毛片久久久久久久女人18 | 国产在线视频一区二区 | 黄色视频网站免费在线观看 | 欧美猛交XXXXX无码 |