| 產(chǎn)品編號 | bs-1373R |
| 英文名稱 | Lipocalin 2/NGAL Rabbit pAb |
| 中文名稱 | 脂質(zhì)運載蛋白抗體 |
| 別 名 | Lipocalin 2; Neutrophil Gelatinase associated Lipocalin; 25 kDa alpha 2 microglobulin related subunit of MMP9; alpha 2 microglobulin related protein; HGNC:6526; HNL; Lcn 2; LCN-2; Lcn2; Neutrophil gelatinase associated lipocalin precursor; NGAL; Oncogene |
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Specific References (4) | bs-1373R has been referenced in 4 publications.
[IF=6.16] Xing, Changhong, et al. "Neuronal Production of Lipocalin-2 as a Help-Me Signal for Glial Activation." Stroke (2014): STROKEAHA-114. IHC-P ; Human.
[IF=4.041] Chen, Yi-Hung. et al. Electroacupuncture improves repeated social defeat stress-elicited social avoidance and anxiety-like behaviors by reducing Lipocalin-2 in the hippocampus. Mol Brain. 2021 Dec;14(1):1-25 IF ; Mouse.
[IF=3.934] Wang Z et al. Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persisted in Adult-aged OffspringEndocrinology.2020 Dec 1;161(12):bqaa164. IHC ; Mouse.
[IF=3.647] Lin D et al. Aldehyde dehydrogenase 2 regulates autophagy via the Akt-mTOR pathway to mitigate renal ischemia-reperfusion injury in hypothermic machine perfusion. Life Sci
. 2020 Jul 15;253:117705. IHC,WB ; mouse.
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| 研究領域 | 腫瘤 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Lipocalin 2: 101-198/198 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances. Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Subunit: Homodimer; disulfide-linked. Heterodimer; disulfide-linked with MMP9. Subcellular Location: Secreted. Note=Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. Tissue Specificity: Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: P80188 Gene ID: 3934 Database links: Entrez Gene: 3934 Human Entrez Gene: 16819 Mouse Omim: 600181 Human SwissProt: P80188 Human SwissProt: P11672 Mouse Unigene: 204238 Human Unigene: 9537 Mouse Unigene: 11303 Rat NGAL在胚胎發(fā)育、細胞分化、細胞凋亡、細胞信號轉(zhuǎn)導、脂質(zhì)代謝及腫瘤的發(fā)生發(fā)展以及參與炎癥免疫反應等多種生命活動過程中發(fā)揮著作用，尤其是近年來在消化系統(tǒng)腫瘤的研究中，受到了重視。 |
| 產(chǎn)品圖片 |
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NGAL Polyclonal Antibody, Unconjugated(bs-1373R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
