| 產品編號 | bs-1088R |
| 英文名稱 | T Beta 10 Rabbit pAb |
| 中文名稱 | 胸腺素β10抗體 |
| 別 名 | MIG 12; MIG12; Migration inducing gene 12; Migration inducing protein 12; Ptmb10; TB 10; TB10; Thyb 10; Thyb10; Thymosin beta-10; Thymosin beta 10; TMSB10; TYB10_HUMAN |
| 研究領域 | 腫瘤 免疫學 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 4.9 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human T Beta 10: 13-110/44 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The beta thymosins are a family of related peptides, initially isolated from calf thymus but known to be present in a wide variety of mammalian and other vertebrate cells and tissues. Thymosin beta 4 was the first member of the family to be characterized. Although TMSB4 was initially proposed to be a thymic hormone acting at early stages of T cell maturation, the high concentration of the protein and its mRNA in a number of other tissues and cells, as well as the lack of an identifiable secretory signal sequence, suggested that it had a general function in many cell types. Thymosin beta 10 is closely related in sequence to TMSB4 and is also an actin sequestering protein. Function: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Subcellular Location: Cytoplasm, cytoskeleton. Similarity: Belongs to the thymosin beta family. SWISS: Q596K9 Gene ID: 9168 Database links: Entrez Gene: 9168 Human Omim: 188399 Human SwissProt: P63313 Human SwissProt: Q596K9 Human Unigene: 446574 Human Unigene: 5983 Rat 胸腺素β10同其他胸腺素一樣也是多肽類激素，具有增強細胞免疫功能和調節(jié)免疫平衡等作用，胸腺素可使由骨髓產生的干細胞轉變?yōu)門淋巴細胞，因而可增強細胞免疫功能，對體液免疫的影響甚微。 近年來研究認為：該蛋白于腫瘤轉移有關，胸腺素β10在腫瘤轉移潛能的增高伴有肌動蛋白多聚體的丟失和微絲結構的解聚,微絲骨架的這種變化與Tβ10的表達增高具有相關性。 |
| 產品圖片 |
Tissue/cell: mouse small intestine; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-T-Beta-10 Polyclonal Antibody, Unconjugated(bs-R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
