| 產(chǎn)品編號 | bs-0026R |
| 英文名稱 | Osteopontin Rabbit pAb |
| 中文名稱 | 骨橋蛋白/分泌型磷蛋白1抗體 |
| 別 名 | BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin. OSTP_Mouse. |
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Specific References (28) | bs-0026R has been referenced in 28 publications.
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| 研究領域 | 神經(jīng)生物學 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:500-2000,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse OPN: 221-280/294 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.
Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma. Subunit: Ligand for integrin alpha-V/beta-3. Subcellular Location: Secreted. Tissue Specificity: Bone. Found in plasma. Similarity: Belongs to the osteopontin family. SWISS: P10923 Gene ID: 20750 Database links: Entrez Gene: 6696 Human Entrez Gene: 20750 Mouse Omim: 166490 Human SwissProt: P10451 Human SwissProt: P10923 Mouse Unigene: 313 Human 骨橋蛋白（osteopontin）又稱為早期Ｔ淋巴細胞活化因子Ｉ或分泌型磷蛋白Ｉ,是一種重要的多功能細胞外基質蛋白,在骨組織細胞、巨噬細胞、平滑肌細胞、內皮細胞及上皮細胞等多種細胞上表達,在介導細胞的趨化、聚集、黏附、增殖和遷移,以及骨組織的礦化與重建、免疫調節(jié)、信號轉導和對感染性疾病的免疫能力等方面有著重要的作用。 ＯＰＮ在細胞免疫中的功能：1.ＯＰＮ對炎性細胞的作用：炎癥是炎性細胞(單核巨噬細胞、淋巴細胞、中性粒細胞等)在炎癥部位的聚集,適度的炎癥對機體有益,如抗感染及促進組織細胞功能恢復等;但過度的炎癥能引起組織損傷等有害作用。ＯＰＮ在炎癥中具有促炎及抗炎雙重作用；2.ＯＰＮ對淋巴細胞的作用：早期的報道稱ＯＰＮ是Ｔ淋巴細胞抑制因子,它可抑制Ｔ淋巴細胞而促進Ｂ淋巴細胞及肥大細胞的分化、增殖 骨橋蛋白(osteopontin，OPN)是一種含精氨酸—甘氨酸—天冬氨酸(Arg-Gly-Asp，RGD)的分泌型糖基化磷蛋白，已歸類于細胞外基質(extracellular matrix，ECM)蛋白。 OPN既是細胞因子，又是細胞黏附分子。 OPN最初是從骨組織分離的，而后在上皮細胞、胃腸道的分泌物、腎、甲狀腺、睪丸以及一些腫瘤細胞里都陸續(xù)的發(fā)現(xiàn)了OPN的表達。在生殖方面，OPN在分泌期子宮內膜上皮細胞、侵襲的滋養(yǎng)細胞、蛻膜細胞中表達。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-Osteopontin (bs-0026R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteopontin) Polyclonal Antibody, Unconjugated (bs-0026R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat shin bone; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated(bs-0026R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated(bs-0026R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (bs-0026R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
