| 產(chǎn)品編號(hào) | bs-0046R |
| 英文名稱 | Neuronal thread protein AD7c-NTP Rabbit pAb |
| 中文名稱 | 神經(jīng)絲蛋白抗體 |
| 別 名 | neuronal thread protein AD7c-NTP; AD7c-NTP. |
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Specific References (1) | bs-0046R has been referenced in 1 publications.
[IF=5.11] He,et al.Disposable Morpho menelaus Based Flexible Microfluidic and Electronic Sensor for the Diagnosis of Neurodegenerative Disease.(2018) Advanced Healthcare Materials. 7:. microfluidic chip ; Human.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neuronal thread protein AD7c-NTP: 301-375/375 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is unusual in that its coding sequence is derived almost entirely from a cluster of different Alu repeat sequences. However, the mRNA and the encoded protein have been shown to be expressed in neurons, and overexpressed in brains with Alzheimer's disease. In vitro studies also demonstrated that abnormal expression of this gene promoted neuritic sprouting and cell death, associated with dementia in Alzheimer's disease. Gene ID: AD7c Database links: GenBank: AAC08737 AD7C-NTP是存在于神經(jīng)元中的一種41Kda的蛋白質(zhì)，在AD患者腦內(nèi)選擇性升高，和其病理過(guò)程相關(guān)，AD7C-NTP基因也只在神經(jīng)元表達(dá)，AD患者腦脊液中AD7C-NTP表達(dá)升高， AD7C-NTP作為AD早期診斷和確診的生物化學(xué)標(biāo)志正引起越來(lái)越多的關(guān)注. |
| 產(chǎn)品圖片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- AD7c-NTP(bs-0046R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Primary: Anti-Neuronal thread protein AD7c-NTP (bs-0046R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 43 kDa
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuronal thread protein AD7c-NTP) Polyclonal Antibody, Unconjugated (bs-0046R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AD7c-NTP Polyclonal Antibody, Unconjugated(bs-0046R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AD7c-NTP Polyclonal Antibody, Unconjugated(bs-0046R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: SHSY5Y.
Primary Antibody (green line): Rabbit Anti-Neuronal thread protein AD7c-NTP antibody (bs-0046R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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