| 產品編號 | bsm-61492R |
| 英文名稱 | Drosha Recombinant Rabbit mAb |
| 中文名稱 | 核糖核酸酶3/Drosha重組兔單抗 |
| 別 名 | Ribonuclease 3(EC:3.1.26.3); Protein Drosha; Ribonuclease III; RNase III; p241; DROSHA; RN3; RNASE3L; RNASEN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 159 kDa |
| 檢測分子量 | 159 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Drosha |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. SWISS: Q9NRR4 Gene ID: 29102 Dircer和Drosha是兩種核糖核酸酶是卵巢癌細胞中RNA干擾機制中的關鍵酶，Dircer和Drosha兩種酶的表達（數(shù)量）越高，存活時間越長。美國科學家研究發(fā)現(xiàn)，卵巢癌患者存活率與兩種酶有關。美國MD安德森癌癥治療中心先后對111個和132個卵巢癌樣本進行了分析。結果發(fā)現(xiàn)，人體內兩種核糖核酸酶Dicer和Drosha含量高的患者平均存活11年，而含量低的患者平均只能活2.86年。Dicer的水平高低對卵巢癌患者尤其重要。這一發(fā)現(xiàn)有助于卵巢癌的診斷和治療。卵巢惡性腫瘤的發(fā)病率在女性常見惡性腫瘤中占2. |
| 產品圖片 |
Western blot analysis of 293 cell lysate. Using Drosha (bsm-61492R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
