| 產(chǎn)品編號 | bsm-61472R |
| 英文名稱 | SFRP1 Recombinant Rabbit mAb |
| 中文名稱 | 分泌型卷曲相關(guān)蛋白1重組兔單抗 |
| 別 名 | Frizzled related protein 1; FRP1; FrzA; SARP2; Secreted apoptosis related protein 2; Secreted frizzled related protein 1; SFRP1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | 35 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FRP 1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. SWISS: Q8N474 Gene ID: 6422 分泌型卷曲相關(guān)蛋白1是近來發(fā)現(xiàn)的新的抑癌基因, 是Wnt信號通道的拮抗通路蛋白,SFRP1能夠阻斷某些發(fā)生在癌癥初期與之相關(guān)的細胞生長基因的信號通路。 有學(xué)者認為：該基因有去甲基化酶的作用，可以阻斷與甲基化進程相關(guān)酶的活性，進而抑制腫瘤基因的改變，SFRP1的作用還有待于更進一步的研究。 |
| 產(chǎn)品圖片 |
Western blot analysis of A375 cell lysate. Using SFRP1 (bsm-61472R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
