| 產(chǎn)品編號(hào) | bsm-61191R |
| 英文名稱 | BBC3/PUMA Recombinant Rabbit mAb |
| 中文名稱 | p53正向細(xì)胞凋亡調(diào)控因子重組兔單抗 |
| 別 名 | BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis; PUMA alpha. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | 18 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BBC3: 150-193 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis.Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis. SWISS: Q9BXH1 Gene ID: 27113 PUMA在由p53誘導(dǎo)而經(jīng)歷細(xì)胞凋亡的細(xì)胞中被p53活化。在線粒體，PUMA 誘導(dǎo)細(xì)胞色素C的釋放和激活對(duì)細(xì)胞凋亡的快速誘導(dǎo)。PUMA 與Bcl2、Bcl-XL、類Bcl2-1蛋白相互作用，促使線粒體易位和BAX的多聚化。PUMA在通過依賴細(xì)胞色素C/APAF1 途徑介導(dǎo)p53誘導(dǎo)的細(xì)胞死亡中也發(fā)揮作用。 |
| 產(chǎn)品圖片 |
Western blot analysis of K562 cell lysate. Using BBC3/PUMA (bsm-61191R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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